Highlights
- •The study revealed a high sero-prevalence of Chlamydia pneumoniae in the studied population.
- •The comparative study between the two methods revealed a significant correlation.
- •We suggest therefore using immunoblot for diagnosis confirmation because it provides additional useful information.
Abstract
Background
Chlamydia pneumoniae is a pathogen associated with human respiratory tract infection,
its viable presence in atherosclerotic plaques is also assumed to play significant
function in cardiac diseases. Our study’s main objective is to evaluate Chlamydia
pneumoniae sero-prevalence in Moroccan patients with cardiovascular diseases using
and comparing two serological methods.
Methods
Two hundred eighteen patients were enrolled; serums were tested by microimmunofluorescence
to explore the sero-prevalence. Simultaneously 74 serums were analyzed by both immunoblot
and micro-immunofluorescence to evaluate recombinant proteins diagnosis value.
Results
MIF results revealed 81% male and 84.5% female positive cases. The comparative study
among 74 patients showed 78% men and 89% women positive cases by immunoblot, whereas
MIF showed respectively 80% and 72%, a significant concordance between these methods
was revealed. However, this comparison showed also two types of discrepancies, which
may be related to difficulties in antigens detection by micro-immunofluorescence resulting
from their structure complexity, or the antibodies reactivity with species’ common
antigens.
Conclusions
The study revealed a high sero-prevalence of Chlamydia pneumoniae in the studied population,
a big interest of recombinant protein was also revealed in the diagnosis accuracy.
We suggest therefore using immunoblot for diagnosis confirmation because it provides
additional useful information.
Keywords
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Article info
Publication history
Published online: December 01, 2018
Accepted:
October 22,
2018
Received in revised form:
August 18,
2018
Received:
May 11,
2018
Identification
Copyright
© 2018 Australasian College for Infection Prevention and Control. Published by Elsevier B.V. All rights reserved.
